Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants

Abstract Mycoplasma pneumoniae is one of the leading causes community-acquired pneumonia in children and adolescents. Because wide application macrolides clinical treatment, macrolide-resistant M. strains have become increasingly common worldwide. However, molecular mechanisms underlying drug resistance are poorly understood. In present work, we analyzed whole proteomes macrolide-sensitive using a tandem mass tag-labeling quantitative proteomic technique, Data available via ProteomeXchange with identifier PXD022220. total, 165 differentially expressed proteins were identified, which 80 upregulated 85 downregulated drug-resistant strain compared sensitive strain. Functional analysis revealed that these predominantly involved protein peptide biosynthesis processes, ribosome, transmembrane transporter activity, implicates them mechanism(s) to macrolides. Our results provide new insights into identify potential targets for further studies on this bacterium.